TLK1b
Protein Name | Human TLK1b (Isoform 3) |
Tag Name |
|
Expression System | E. coli Rosetta-gami™ 2 (DE3)pLysS |
Accession Number |
|
Product Description | Tousled-like kinase 1B (TLK1B) is a splice variant of the serine/threonine kinase TLK1, involved in DNA replication, repair, and chromatin remodeling. It plays a crucial role in maintaining genome stability, particularly during replication stress. TLK1B is activated in response to cellular stress and has been implicated in cancer progression due to its role in promoting survival and proliferation of tumor cells. |
Formulation |
|
Specific Activity |
|
Storage and Stability | 20ºC for long term, 4ºC for short term use |
Handling Recommendations | Proteins should be stored at cold temperatures when not in use. To minimise freeze-thaw cycles, aliquot the protein into smaller portions of at least 10 μL to prevent evaporation and maintain consistent concentration. |
Quality Control Testing
Kinase assay protocol
1X Kinase reaction Buffer (KRB): 40 mM Tris-Cl (pH=7.5), 20 mM MgCl2, 2.5 % Glycerol, 0.1 mg/ml BSA
The desired concentration of TLK1B (2X)- 2µM
Substrate dilution (2X): 2 µM in 1X KRB
ATP dilution (2X): 200 µM in 1X KRB
Assay Procedure
1. The ADP-Glo kinase assay was performed in a solid, white, flat bottom 96-well plate.
2. The reaction volume of 20 µl with 2X 1 µM kinase, 2X 1 µM kinase substrate, and 2X 100 µM ATP in 1X Kinase Reaction Buffer (KRB).
3. The reaction was incubated for an hour at 30°C with gentle shaking.
4. 20 µL of ADP-GLo reagent is added and incubated for 45 minutes.
5. 40 µl of a Kinase Detection Reagent is added to each well and incubated for 60 minutes in the dark.
6. The readout was measured as luminescent values (Relative luminescent unit, RLU).
English (UK) 
English (United States)